c5ar antagonist w54011 Search Results


c5ar antagonist w54011  (Merck KGaA)
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Merck KGaA c5ar antagonist w54011
Transcriptome sequencing of NSCLC tissues, the expression of KLF5, GCN5, GDF15, <t>C5aR</t> in tumor tissues and the content of C5a in plasma of NSCLC patients. a Venn diagram showed that a total of 944 genes were upregulated (≥2-fold) by RNA-seq of 12 pairs of NSCLC tissues and 86 genes were in the overlap of all three sequencing results. b Gene ontology (GO) analysis for the 86 genes displayed that represented biological processes included cell proliferation, growth and maintenance. c , d Representative images of IHC staining for KLF5, GCN5, and GDF15 in paraffin-embedded sections of NSCLC patients ( c ) and statistical analysis of IHC staining intensity and area ( d ). The expression of KLF5, GCN5, and GDF15 in NSCLC tumor tissues were increased compared to adjacent tissues ( N = 185). e Quantification of plasma C5a in 40 NSCLC patients and 40 healthy volunteers by ELISA. Plasma C5a level of NSCLC patients was higher than control donors (** P < 0.01). f , g Representative result ( f ) and statistical analysis ( g ) for C5aR expression in NSCLC tissues by IHC. The C5aR expression was upregulated in NSCLC tumor tissues in comparison with adjacent tissues ( N = 185)
C5ar Antagonist W54011, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Transcriptome sequencing of NSCLC tissues, the expression of KLF5, GCN5, GDF15, C5aR in tumor tissues and the content of C5a in plasma of NSCLC patients. a Venn diagram showed that a total of 944 genes were upregulated (≥2-fold) by RNA-seq of 12 pairs of NSCLC tissues and 86 genes were in the overlap of all three sequencing results. b Gene ontology (GO) analysis for the 86 genes displayed that represented biological processes included cell proliferation, growth and maintenance. c , d Representative images of IHC staining for KLF5, GCN5, and GDF15 in paraffin-embedded sections of NSCLC patients ( c ) and statistical analysis of IHC staining intensity and area ( d ). The expression of KLF5, GCN5, and GDF15 in NSCLC tumor tissues were increased compared to adjacent tissues ( N = 185). e Quantification of plasma C5a in 40 NSCLC patients and 40 healthy volunteers by ELISA. Plasma C5a level of NSCLC patients was higher than control donors (** P < 0.01). f , g Representative result ( f ) and statistical analysis ( g ) for C5aR expression in NSCLC tissues by IHC. The C5aR expression was upregulated in NSCLC tumor tissues in comparison with adjacent tissues ( N = 185)

Journal: Oncogene

Article Title: C5a induces A549 cell proliferation of non-small cell lung cancer via GDF15 gene activation mediated by GCN5-dependent KLF5 acetylation

doi: 10.1038/s41388-018-0298-9

Figure Lengend Snippet: Transcriptome sequencing of NSCLC tissues, the expression of KLF5, GCN5, GDF15, C5aR in tumor tissues and the content of C5a in plasma of NSCLC patients. a Venn diagram showed that a total of 944 genes were upregulated (≥2-fold) by RNA-seq of 12 pairs of NSCLC tissues and 86 genes were in the overlap of all three sequencing results. b Gene ontology (GO) analysis for the 86 genes displayed that represented biological processes included cell proliferation, growth and maintenance. c , d Representative images of IHC staining for KLF5, GCN5, and GDF15 in paraffin-embedded sections of NSCLC patients ( c ) and statistical analysis of IHC staining intensity and area ( d ). The expression of KLF5, GCN5, and GDF15 in NSCLC tumor tissues were increased compared to adjacent tissues ( N = 185). e Quantification of plasma C5a in 40 NSCLC patients and 40 healthy volunteers by ELISA. Plasma C5a level of NSCLC patients was higher than control donors (** P < 0.01). f , g Representative result ( f ) and statistical analysis ( g ) for C5aR expression in NSCLC tissues by IHC. The C5aR expression was upregulated in NSCLC tumor tissues in comparison with adjacent tissues ( N = 185)

Article Snippet: The recombinant human C5a was from R&D systems (Tustin, CA, USA), and C5aR antagonist W54011 was provided by Merck Millipore (Darmstadt, Germany).

Techniques: Sequencing, Expressing, Clinical Proteomics, RNA Sequencing, Immunohistochemistry, Enzyme-linked Immunosorbent Assay, Control, Comparison

C5aR expression in human NSCLC cell lines as well as cell proliferation and KLF5, GCN5, GDF15 expression upon C5a stimulation. a IB assay showed that human C5aR was highly expressed in A549 and PC9 cell lines. b – d The proliferation of A549 and PC9 cells exposed to C5a (25 ng/ml and 250 ng/ml, especially 25 ng/ml) was notably increased by CCK8 (72 h, b ), cell counting (7 days, c ), and colony-forming assays (14 days, d ). e , f Real-time PCR ( e ) and IB ( f ) exhibited that mRNA and protein of KLF5, GCN5, and GDF15 in A549 cells following 25 ng/ml C5a incubation started to increase from 1 h, and peaked at 2 h and 3 h (GCN5 peaked at 6 h). Representative photographs are manifested. Data are expressed as means ± S.E.M. from three independent experiments, * P < 0.05, ** P < 0.01 vs. 0 ng/ml C5a

Journal: Oncogene

Article Title: C5a induces A549 cell proliferation of non-small cell lung cancer via GDF15 gene activation mediated by GCN5-dependent KLF5 acetylation

doi: 10.1038/s41388-018-0298-9

Figure Lengend Snippet: C5aR expression in human NSCLC cell lines as well as cell proliferation and KLF5, GCN5, GDF15 expression upon C5a stimulation. a IB assay showed that human C5aR was highly expressed in A549 and PC9 cell lines. b – d The proliferation of A549 and PC9 cells exposed to C5a (25 ng/ml and 250 ng/ml, especially 25 ng/ml) was notably increased by CCK8 (72 h, b ), cell counting (7 days, c ), and colony-forming assays (14 days, d ). e , f Real-time PCR ( e ) and IB ( f ) exhibited that mRNA and protein of KLF5, GCN5, and GDF15 in A549 cells following 25 ng/ml C5a incubation started to increase from 1 h, and peaked at 2 h and 3 h (GCN5 peaked at 6 h). Representative photographs are manifested. Data are expressed as means ± S.E.M. from three independent experiments, * P < 0.05, ** P < 0.01 vs. 0 ng/ml C5a

Article Snippet: The recombinant human C5a was from R&D systems (Tustin, CA, USA), and C5aR antagonist W54011 was provided by Merck Millipore (Darmstadt, Germany).

Techniques: Expressing, Cell Counting, Real-time Polymerase Chain Reaction, Incubation